Author Archives: aalexiev

About aalexiev

I am a third year Microbiology major with a minor in Evolution and Ecology at UC Davis.

Learning How to Work Qiime

So we’ve done our data collection, we’ve done more PCR than we could ever imagine, and we finally got our sequences. Now what? We analyze our data using Qiime, a software that will help us see connections between our microbe … Continue reading

Posted in Undergraduate Aquarium Project | 2 Comments

Finally back in the lab

After spending the majority of our time collecting samples and doing water chemistry on site, we are all back in our lab ready to do DNA extractions and PCR galore. Unfortunately, the kim wipes we used to scrub microbes off … Continue reading

Posted in Undergraduate Aquarium Project | 2 Comments

Sample testing using new equipment

We finally got all our equipment for gathering metadata on the water, and decided to do some sample testing using tap water and saltwater from the tank. We ended up with the following:   Tap water – NO2-N levels: 65 … Continue reading

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New Parameters to Test!

We’ve ordered and are recieving a series of equipment to measure Nitrate, Nitrite, Ammonia, dissolved Oxygen, pH, salinity, temperature, Phosphate, alkalinity, Chloride, hardness, Iron, and Sulfite in the tanks’ waters. Now we can gather more information about the environment these microbes … Continue reading

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Sampling, DNA extraction, and PCR, OH MY!

For the past week, we’ve been doing preliminary sampling, as well as some DNA extractions and PCR on samples from the saltwater and freshwater flasks of water that we filtered and the wall and protein skimmer from the saltwater tank. … Continue reading

Posted in Undergraduate Aquarium Project | 2 Comments

Introductory Post

Yes, a boring title, but that’s exactly what this is. I am Alex Alexiev, second year biological sciences major. I’ve been thinking about a microbiology major for the past year or so, and this lab seems like a step in … Continue reading

Posted in Undergraduate Aquarium Project | 1 Comment