Category Archives: Undergraduate Aquarium Project

Today was the final presentation by the students working on the aquarium research project. Alex, Lakshmi, Sabreen, Andrew, and Kevin all talked about their recent work using QIIME to analyze the 16S sequence data from the project. Their presentations, along … Continue reading →

So we’ve done our data collection, we’ve done more PCR than we could ever imagine, and we finally got our sequences. Now what? We analyze our data using Qiime, a software that will help us see connections between our microbe … Continue reading →

Seven months after starting the sample collections we finally submitted our pooled 16S library today for sequencing.  We have somewhere around 90 samples from a few different types of aquariums and our community succession experiment.  Now time for everyone to … Continue reading →

I’m sitting here in the lab looking at our google doc and it’s looking pretty good! We only have a few final dilutions to do and we’ll be on our way to sequencing! I’m waiting for David to come back … Continue reading →

A few of the PCRs last week didn’t work so I’m finishing up the last PCR B’s right now that we’ve had to redo and then all we have to do is Gel confirmations. If I counted correctly there are … Continue reading →

Here’s the poster that Lakshmi prepared for the conference.   The presentation went well!  Thanks to everyone who helped out.

Today I did 32 PCR B’s. It may sound like a lot but PCR B’s are extremely easy so it went by pretty quick. I also messed up on updating the google doc and had to throw away three PCR … Continue reading →

Today I primarily focused on reorganizing some of our stuff. First, I went through our two boxes of extracted DNA and put them in numerical order by sample ID. I think at one point they were in order but with … Continue reading →

I’ve spent the week doing PCR A! So far I’m up to 40+ PCR A reactions. I finished up the 20 remaining samples needing PCR A this morning. After losing track of where I was in the PCR A process … Continue reading →